The Effect of Incorporating Nanocalcium Phosphate Particles into Biodentine on Pulpal Tissue Response (In Vitro Study)

Document Type : Original Article

Authors

1 Assistant lecturer of Operative Dentistry, Faculty of Dentistry, Ahram Canadian University (ACU).

2 Professor of Operative Dentistry, Faculty of Dental Medicine for Girl’s, AL-Azhar University.

3 Professor of Operative Dentistry, and Dean of Faculty of Dental Medicine Sinai University.

Abstract

Objectives: This study was conducted to evaluate the effect of incorporating Nanocalcium phosphate (NCP) particles into Biodentine™ on pulpal tissue response as a direct pulp capping material (entire tooth culture model). Materials and methods: - A total of forty freshly human premolar teeth extracted for orthodontic reasons were used. After cavity preparation and mechanical pulp exposure, the teeth were divided into 2 main groups (20 teeth each) according to the capping material used; where group (M1) refers to pulps capped with Biodentine™, and group (M2) refers to pulps capped with Biodentine incorporated with NCP. Each group was further subdivided into two subgroups according to the culture period; where subgroup (P1) refers to teeth cultured for 14 days, and subgroup (P2) refers to teeth cultured for 28 days. At the end of each culture period, the teeth were histologically examined to assess the dentine bridge thickness of each group after 14 and 28 days. Moreover, elemental analysis of Calcium and Phosphorous ions in the dentine bridge was performed using SEM-EDX. Results: Direct pulp capping with Biodentine incorporated with NCP showed thicker dentine bridge when compared to Biodentine™ alone. There is a positive correlation between the weight % of Calcium and Phosphorous ions in the dentine bridge below both capping materials and the dentine bridge thickness. Conclusion: Biodentine incorporated with NCP could be a good candidate for pulp capping in the future

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Al-Azhar Dental Journal for Girls
Volume 5, Issue 2
April 2018
Pages 181-186

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