A Comparative Study of the Antibacterial Efficacy of two Natural Irrigating Solutions with Two Different Root Canal Sealers on E-feacalis

Nour El Deen, Noha; Kamel, Wael; Sherief, Mohamed; Rokaya, Mohammed

doi:10.21608/adjg.2017.5286

A Comparative Study of the Antibacterial Efficacy of two Natural Irrigating Solutions with Two Different Root Canal Sealers on E-feacalis

Document Type : Original Article

Authors

¹ Dentist at the Ministry of Health.

² Professor and Head of Endodontics Department, Faculty of Dental Medicine (for girls) Al-Azhar University.

³ Professor of Microbiology, Faculty of Medicine, Al-Azhar University

⁴ Lecturer of Endodontics, Faculty of Dental Medicine, Al-Azhar University- Assiut Branch.

10.21608/adjg.2017.5286

Abstract

This study aimed to: evaluate the antibacterial efficacy of two natural irrigating solutions
(Green Tea, and Propolis) with two different root canal sealers (AH Plus sealer,
and Total Fill BC Sealer) against E. faecalis. Methods: One hundred single rooted
teeth were selected. All root canals were prepared manually to #50 file. Finally the
teeth were sterilized by gamma rays. Each root canal was inoculated with 0.5 ml of E.
faecalis. Specimens were divided randomly into two main groups (50 specimens each)
according to the irrigating solution; A1 (Green Tea), and A2 (Propolis). Each main
group was divided into two subgroups with (25 specimens each) according to the type
of sealer used B1 (AH Plus sealer), and B2 (TotalFill BC Sealer). The bacterial sampling
counting process was done three times throughout the study. Five specimens from
each subgroup were examined by SEM for presence or absence of E. faecalis. Results:
The highest mean value of bacterial count was found in (Green tea) (6.50x10x10 ±
1.29x10x10) while the least mean value of bacterial count was found in (Propolis) (with
less antibacterial effect 2x10x10 ± 1.33x10x10) with higher antibacterial effect. The
highest mean value of bacterial count was found in (AH Plus Sealer) (5.40x10x10 ±
2.44x10x10) with less antibacterial effect while the least mean value of bacterial count
was found in (TotalFill BC Sealer) (3.10x10x10 ± 1.54 x10x10) with higher antibacterial
effect. Conclusions: Propolis is an effective intracanal irrigant in eradicating E.
faecalis. On other hand TotalFill BC Sealer is more effective in reducing E. faecalis.