The Effect of using Cross-Linking Agent with Nano-Adhesive on the Bonding to Sound and Caries Affected Dentin

Document Type : Original Article


1 Lecturer of Operative Dentistry, Faculty of Oral and Dental Medicine, Nahda University

2 Professor and head of Operative Dentistry Department, Faculty of Dental Medicine Al-Azhar University, Girls Branch

3 Professor of Operative Dentistry, Faculty of Oral and Dental Medicine, Cairo University

4 Associate Professor of Operative Dentistry, Faculty of Oral and Dental Medicine, Cairo University


Aim: This study was designed to evaluate the microtensile bond strength and the effect of storage on using two different concentrations (6.5% and 15 %) of grape seed extract cross-linking agent with nano-adhesive single bond universal adhesive to sound and caries affected dentin. Methods: A total of sixty molars was divided into 2 main groups (30 each) according to the type of substrate either sound or caries affected dentin. Each group was divided into three equal subgroups (10 teeth each) according to the treatment with cross linking agent (Grape seed extract (GSE)), either with no treatment with GSE or 6.5% or 15% GSE dissolved in ethyl alcohol anhydrous. Each subgroup was further subdivided into 2 equal subgroups according to the storage period (5 teeth each) either after 24 hours or after 3 months. Resin composite core was built up and then specimens were sectioned to obtain composite-dentin slabs with a cross-sectional area of approximately 1mm2. They were subjected to microtensile bond strength testing at a cross head speed of 1.0mm/min. Statistical analysis was done using three way-ANOVA followed by post hoc test for comparison (P≤ 0.05). Results: The highest microtensile bond strength mean value was recorded for sound dentin and caries affected dentin, specimens treated with 6.5% grape seed extract after 24 hours and 3 months storage period, followed by specimens treated with 15% grape seed extract, while specimens without grape seed extract treatment showed the lowest significant microtensile bond strength mean value. Conclusions: using cross linking agent is considered a beneficial way in improving bonding to sound and caries affected dentin.6.5%concentration of grape seed extract as a cross linking agent is the concentration of choice to be used. Time has a deteriorating effect on the formed resin -dentin bond.