Effect of Protein Repellent Addition to Self-Healing Experimental Resin Composite on Microcosm Biofilm and Cytotoxicity

Document Type : Original Article

Authors

1 Associate Lecturer of Dental Biomaterials, Al Zagazig University, Egypt

2 Professor of Dental Biomaterials, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt

3 Professor in department of Polymer and Pigment chemistry, National Research Center, Cairo, Egypt

4 Professor of Microbiology, Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt

Abstract

Purpose: To investigate the effect of addition of MPC protein repellent agent to selfhealing
experimental resin composite on the self-healing efficacy, microcosm biofilm
and the cytoxicity. Material and Methods: Polyurea formaldehyde microcapsules
were synthesized by Insitu Polymerization Technique. MPC and the microcapsules are
added to the prepared experimental resin composite with different MPC concentrations.
Protein repellence essay was examined by the Micro bicinchoninic Acid method.
Biofilm models formed of streptococcus mutans growth was prepared and visualized by
confocal laser electron microscope to investigate the live \ dead assay over the studied
specimens. The cytotoxicity of the specimens was examined via SRB assay. Fracture
toughness was examined using universal testing machine till fracture. Self-healing
efficacy is evaluated. Results: In protein deposition, it was found that the 0%MPC
has the highest value while the least value was found in the 10% group. In live/
dead bacterial assay, it was found that the 0%MPC has the highest living bacterial
value and the lowest dead bacterial value while the 7.5% group has the lowest living
bacterial value and the highest dead bacterial value. In the mechanical evaluation,
the virgin fracture toughness, the highest facture toughness was found in the control
specimens while the least fracture toughness was found in 10% group, the selfhealing
efficacy results showed that the highest self-healing value was found in the
0%MPC while the least self-healing efficacy value was found in the control group.
Conclusion: Innovative self-healing composite with protein repellence give rise for
a great variety of dental biomaterials with more promising results and long clinical
serviceability
 

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Volume 10, Issue 2
Issue2 April-Restorative Dentistry issue (Removable Prosthodontics, Fixed Prosthodontics, Endodontics, Dental Biomaterials, Operative Dentistry)
April 2023
Pages 325-334