Document Type : Original Article
Authors
1
Assistant lecturer in Oral Medicine, Periodontology, Oral Diagnosis and Dental Radiology Department. Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
2
Professor of Oral Medicine, Periodontology, Diagnosis and Radiology Department, Faculty of Dental Medicine for Girls, Al-Azhar University, Cairo, Egypt.
3
Professor of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
4
Professor of bioinformatics, Biology Department, school of science and Engineering, American University in Cairo, Egypt
Abstract
Purpose: This study was performed for identification and prevalence of periodontal microbiota in a group of Egyptians with a special emphasis on the correlation between different periodontal stages and molecular bacterial identification. Subjects and methods: 100 Subjects were divided equally into five groups with reference to new classification of periodontitis. Clinical parameters including probing pocket depth (PPD), clinical attachment level (CAL) gingival index (GI) and plaque index (PI) were measured. Samples of subgingival plaque were taken for microbiological analysis and bacterial DNA extraction. Bacterial identification was done using polymerase chain reaction (PCR) for 5 bacteria (Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Treponemadenticola (Td), Tannerellaforsythia (Tf), Fusobacterium nucleatum (Fn) in all samples for the study population. Results: The mean plaque index, gingival index, PD, and CAL were calculated, and the prevalence of P.g, T.d, T. f,A.a and F.n was also identified. Conclusion: for all kinds of bacteria the mean of real time increased by increasing the stage number. The difference between the healthy group and all stages was statistically significant. We can arrange the bacteria sequence as follows based on the overall mean of real time PCR for each type of bacteria: T.f,P.g, T.d,F.n,A.a. It was also clear that the relationship between PD and CAL and other bacterial microbiota was statistically significant.
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