Evaluation of Antimicrobial Efficacy of Nano Chitosan and Chlorohexidine versus Sodium Hypochlorite as Final Rinse against Enterococcus Faecalis

Document Type : Original Article

Authors

1 Dental Practitioner in Governmental Medical Center in El Sherouk City

2 Professor and head of Endodontic Department Faculty of Dental Medicine, Future University, Professor of Endodontic Department Faculty of Dental Medicine for Girls Al-Azhar University, Cairo, Egypt.

3 Associate Professor and head of Endodontic Department Faculty of Dentistry, Horus University, Egypt, Associate Professor of Endodontic, Endodontic Department Faculty of Dental Medicine, Al-Azhar University- Assiut Branch, Egypt.

4 Professor of Microbiology and Immunology Faculty of Medicine, Al-Azhar University, Cairo, Egypt.

Abstract

Purpose: This study aimed to evaluate the antimicrobial efficacy of nano chitosan and chlorohexidine versus sodium hypochlorite as final rinse against Enterococcus faecalis. Methods: Forty single rooted teeth were selected. All root canals were prepared manually to #50 file. Finally, the teeth were sterilized by gamma rays. Each root canal was inoculated with 0.5 ml of E. faecalis. Specimens were divided randomly into 4 main groups (10 specimens each) according to the irrigating solutions. Group 1: nano chitosan 0.2%, group 2: Chlorhexidine 2%, group 3: alternating solution of nano chitosan 0.2% & Chlorhexidine 2%, and group 4: Sodium hypochlorite 3%. The mean of bacterial count before and after final irrigation by the tested solutions were measured. Results: The highest mean difference value of bacterial count was found in 3% sodium hypochlorite, followed by alternating solution of 0.2% nano chitosan & 2% chlorhexidine, then 2% chlorhexidine, and 0.2% nano chitosan came lastly. However, there was no statistically significant difference among the tested groups. Conclusions: alternating solution of 0.2% nano chitosan & 2% chlorhexidine when used as antibacterial irrigant gives promising results and can be used as an alternative to NaOCl solution.

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Al-Azhar Dental Journal for Girls
Volume 10, Issue 1
Issue1 January-Restorative Dentistry issue (Removable Prosthodontics, Fixed Prosthodontics, Endodontics, Dental Biomaterials, Operative Dentistry)
January 2023
Pages 11-17

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