Potential Effect of Alendronate on Tooth Eruption and Molar Root Formation in Young Growing and Osteoprotic Albino Rats (A Histological and Histochemical Study)

Document Type : Original Article

Authors

1 Demonstrator of Oral and Dental Biology, Faculty of Dental Medicine for Girls , Al- Azhar University.

2 Professor and Head of Oral and Dental Biology Department, the Former Dean of Faculty of Dental Medicine for Girls, Al- Azhar University.

3 Assistant Professor of Oral and Dental Biology, Faculty of Dental Medicine for Girls, Al- Cairo University.

Abstract

Aim:
The aim of the present study to evaluate the potential effect of Sodium Alendronate on tooth eruption and root formation in young growing and osteoporotic
albino rats .
 
Materials and Methods Sixty newborn wister albino rats with their average weight 6 grams, each were used in the study. Rats were divided into 4 groups (groupI, groupII, group III &group IV) : GI negative control, GII positive control, GIII Alendronat treated group, GIV (alendronate + dexamethasone) then each group was divided into 3 subgroups according to the date   of scarification at7,14,30 days. Rats were subjected to subcutaneous injection of sodium alendronate and dexamethasone in their dorsal back. The specimens were processed for histological and histochemical analysis.
Results:
alendronate prevented tooth eruption and root formation but dexamethasone caused resorption of bone over developing molar but didn’t affect molar eruption and root formation.
 
Conclusion: sodium alendronate caused increase in bone formation and obstructed eruption pathway. Sodium alendronate prevented maturation of alveolar bone while dexamethasone caused destruction of alveolar bone but didn’t affect normal tooth eruption.

Keywords

Main Subjects


1.
 
Marks SC Jr and Schroeder HE, Tooth eruption: theories
and facts. Anat Rec 1996; 374–393
2. G. Sandgren, “GAPO syndrome: a new case,” American
Journal of Medical Genetics 1995; 87–90
3.
 
Rhen T and Cidlowski JA. Anti inflammatory action of
glucocorticoids--new mechanisms for old drugs. The New
England journal of medicine 2005; 1711-23
 
4. Chrousos GP and Kino T. Intracellular glucocorticoid
signaling: a formerly simple system turns stochastic.
Science’s STKE: signal transduction knowledge
environment 2005; 48-66
5. Harris ST, Watts NB, Jackson RD et al. Four-year study of
intermittent cyclic etidronate treatment of postmenopausal
osteoporosis: three years of blinded therapy followed by
one year of open therapy. Am J Med 1993; 557–567
6. Asikaran S. Bisphosphonates: an overview with special refer
 
ence
to alendronate. Ann ClinBiochem, 2001; 608–23
7. Massa LF, Bradaschia-Correa V, Arana-Chavez VE
Immunocytochemical study of amelogenin deposition during
the early odontogenesis of molars in alendronate-treat
 
ed
newborn rats. J Histochem Cytochem 2006; 713–725
8. Brown & Richard. Histologic Preparations: Common
Problems and Their Solutions. College of American
Pathologists, 2009; 95-101
9.
 
Carson, Freida L., and Christa Hladik. Histotechnology:
A Self Instructional Text. 3
 
rd ed.: American Society of
Clinical Pathologists, 2009; 162-165
10. Gary E. Wise, Robert L. Grier IV, Steven J. Lumpkin,
Quiyang Zhang. Effects of Dexamethasone on Tooth
Eruption in Rats: Differences in Incisor and Molar
Eruption Clin. Anat. 2001; 204–209
11.
 
McClung M. Use of highly potent bisphosphonates in the
treatment of osteoporosis. Curr Osteoporos 2003;116–122
12.
 
Maasalu K, Haviko T, Martson A. Treatment of children
with osteogenesis imperfecta in Estonia. Acta Paediatr
2003; 452–455
13. van Beek ER, Cohen LH, Leroy IM, Ebetino FH, Löwik
CWGM, Papapoulos SE. Differentiating the mechanisms of
resorptive action of nitrogen containing bisphosphonates.
Bone, 2003; 805–811
14. Grier RL & Wise GE. Inhibition of tooth eruption in the rat
by a bisphosphonate. J Dent Res 1998; 8–15
15.
 
Marks SC Jr &Schroeder HE. Tooth eruption: theories and
facts. Anat Rec 1996; 374–393
16.
 
Wise GE, Frazier-Bowers S, D’Souza RN. Cellular,
molecular and genetic determinants of tooth eruption. Crit
Rev Oral Biol Med 2002; 323–334
17. (105) Marks SC Jr. Pathogenesis of osteopetrosis in the
IA rat: reduced bone resorption due to reduced osteoclast
function. Am J Anat 1973; 165–189